OBJECTIVES: After kidney transplantation, human BK polyomavirus (BKPyV) can induce a progressive disease, in three stages: viruria, viraemia, and then nephropathy after a few months of viral replication. Therapeutic intervention is recommended when BKPyV is detected in the plasma.

Chapter 12: Urine the cytologic impression that these cells are infected with the human polyoma virus. (Voided urine, SV-40 immunoperoxidase stain, 630x)

However, little is known of the dynamics of polyomavirus excretion in the urine of healthy adults. Using decoy cells as a marker of polyomavirus viruria cytology has a sensitivity of 41.9% and negative predictive value of 82.8%. The specificity and positive predictive value for viruria (not viral nephropathy) are 100%. False-negative results occurred in samples with suboptimal cellularity, vaginal contamination, and a low viral load. One patient with a false-negative urine cytology developed BKV nephropathy on follow-up. BK polyomavirus in the urine for follow-up of kidney transplant recipients.

Human polyomaviruses are emerging pathogens that infect a large percentage of the human population and are excreted in urine. Consequently, urine that is collected for fertilizer production often has high concentrations of polyomavirus genes. Urine cytology is technically the simplest method to monitor polyomavirus infection after transplantation. Transient shedding of virus-infected cells can be seen in acute cellular rejection, tacrolimus nephrotoxicity, and acute tubular necrosis. A morphologic sign of the (re) activation of polyomaviruses is the detection of typical intranuclear viral inclusion bearing epithelial cells, so-called “decoy cells”, in the urine. Decoy cells often contain polyoma-BK-viruses. 2016-08-12 · Human Polyomaviruses (HPyVs) are small, non-enveloped double-stranded DNA viruses.

The BK virus is a member of the polyomavirus family. Past infection with the BK virus is widespread, but significant consequences of infection are uncommon, with the exception of the immunocompromised and the immunosuppressed.BK virus is an abbreviation of the name of the first patient whom the virus was isolated from in 1971 (the patient was then 29 years old).

We compared the relative efficacy of exfoliative cytology and polymerase chain reaction (PCR) for detecting viruria in 100 urine samples. Definite decoy cells were seen in 8% and probable decoy cells in 5% of specimens. 2016-08-12 · Human Polyomaviruses (HPyVs) are small, non-enveloped double-stranded DNA viruses. They are found in urine, feces and wastewater as a result are used as sewage source tracking viruses and are emerging as potential environmentally related transmission of viral induced cancer.

The BK virus is a member of the polyomavirus family. Past infection with the BK virus is widespread, but significant consequences of infection are uncommon, with the exception of the immunocompromised and the immunosuppressed. BK virus is an abbreviation of the name of the first patient whom the virus was isolated from in 1971 (the patient was

False-negative results occurred in samples with suboptimal cellularity, vaginal contamination, and a low viral load. One patient with a false-negative urine cytology developed BKV nephropathy on follow-up. BK polyomavirus in the urine for follow-up of kidney transplant recipients. Brochot E (1), Descamps V (2), Handala L (2), Faucher J (3), Choukroun G (3), Helle F (4), Castelain S (2), François C (2), Duverlie G (2), Touzé A (5). (1)Department of Virology, Amiens University Medical Centre, Amiens, France; AGIR Research Unit, EA4294, Jules Verne University of Picardie, Amiens, France. Two polyomaviruses, BK virus (BKV) and JC virus (JCV), are ubiquitous in the human population, generally infecting children asymptomatically and then persisting in renal tissue.

This test, previously designated as a Breakthrough Device by the FDA and cleared for use with ethylenediaminetetraacetic acid (EDTA) plasma samples to aid in the Randhawa P, Vats A, Shapiro R: Monitoring for polyomavirus BK And JC in urine: comparison of quantitative polymerase chain reaction with urine cytology. Transplantation.
Chromogenics avanza

detecting Polyomavirus BK and JC in urine specimens. Sensitivity: For each pathogen target, a positive vector control containing the amplification target was generated, serially diluted, and added as template DNA in duplicate.

BK virus (BKV) tillhör släktet polyomavirus för BKV DNA med PCR i urin eller plasma/serum under de polyomavirus DNA in undiluted urine from.
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A morphologic sign of the (re) activation of polyomaviruses is the detection of typical intranuclear viral inclusion bearing epithelial cells, so-called “decoy cells”, in the …

Clinical monitoring for polyomavirus infection is becoming common, but the optimal detection technique remains undefined. We compared the relative efficacy of exfoliative cytology and polymerase chain reaction (PCR) for detecting viruria in 100 urine samples. Definite decoy cells were seen in 8% and probable decoy cells in 5% of specimens.